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Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

DOI :

10.3791/52076-v

March 23rd, 2015

March 23rd, 2015

38,591 Views

1Chemistry Department, Elon University, 2Department of Neurobiology and Anatomy, Wake Forest School of Medicine, 3Neuroscience Graduate Program, Wake Forest School of Medicine, 4ALS Center Translational Science Unit, Wake Forest School of Medicine

Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.

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Keywords Mitochondria Isolation

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