April 1st, 2011
•This protocol describes a method for real-time measurement of mitochondrial calcium fluxes by fluorescent imaging. The method takes advantage of a circularly permutated YFP-based dual-excitation ratiometric calcium sensor (ratiometric pericam-mt) selectively expressed in mitochondria.
Tags
Related Videos
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
Calcium Imaging of Cortical Neurons using Fura-2 AM
Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
Measuring Fast Calcium Fluxes in Cardiomyocytes
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved