Collection of Extracellular Enzymes from Hemolymph
2:13
Collection of Extracellular Enzymes from Midgut Juice
3:31
dsRNA Degradation Assay
5:31
Oral Administration of dsRNA
6:42
Results: Knock Down of Tubulin Expression in the Midgut
7:38
Conclusion
Transcript
The overall goal of this protocol is to deplete gene expression in the gut of cockroaches using RNA interference via oral ingestion of double-stranded RNA encapsulated in liposome carriers. This method can help answer key questions in the developm
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This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.