Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
5:21
Flow Cytometry
7:30
Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
8:31
Conclusion
Transcript
The overall goal of this experiment is to determine the ability of adjuvants of interest to generate cytotoxic T lymphocytes by monitoring adoptively transferred CFSE stained OT1 CD8 positive T cell proliferation in the draining lymph nodes and sp
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We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.