Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

10.0K Views

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07:04 min

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September 16th, 2018

DOI :

10.3791/57640-v

September 16th, 2018

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Jun-O Jin¹^,², Soyeong Jang³, Hyehyun Kim⁴, Junghwan Oh⁴, Sungbo Shim⁵, Minseok Kwak³^,⁴, Peter C.W. Lee⁶

¹Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, ²Department of Medical Biotechnology, Yeungnam University, ³Department of Chemistry, Pukyong National University, ⁴Marine-integrated Bionics Research Center, Pukyong National University, ⁵Department of Biochemistry, Chungbuk National University, ⁶Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

Summary

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Keywords Immunostimulatory Agent

Lymphoid Tissue

Dendritic Cell Activation

Mediastinal Lymph Node

DNA Amphiphile

Nano sized Micelles

Toll like Receptor 9

Immune stimulating Nanoparticle INP

Injection Route

Immunological Analysis

Spleen

Lymph Nodes

Chapters in this video

0:00

Title

0:16

Amphiphilic Oligodeoxyribonucleotide to form DNA Nanoparticles

1:44

Administration of INP via Multiple Injection Routes

2:56

Isolation of Lymph Nodes and Spleen

6:06

Results and Conclusion

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