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Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

DOI :

10.3791/57401-v

June 19th, 2018

June 19th, 2018

8,411 Views

1Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research

We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.

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Keywords OT1 CD8 T Cells

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