JoVE Logo
Faculty Resource Center

Sign In

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

DOI :

10.3791/57440-v

9:33 min

May 3rd, 2018

May 3rd, 2018

7,820 Views

1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute, University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute, University of Calgary

Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.

Tags

Automated Slide Scanning

-- Views

Related Videos

article

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

article

Video-rate Scanning Confocal Microscopy and Microendoscopy

article

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

article

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

article

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

article

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

article

Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

article

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

article

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

article

Electric and Magnetic Field Devices for Stimulation of Biological Tissues

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved