JoVE Logo
Faculty Resource Center

Sign In

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

DOI :

10.3791/50047-v

May 3rd, 2013

May 3rd, 2013

17,613 Views

1Department of Biochemistry and Molecular Medicine, University of California, Davis , 2NSF Center for Biophotonics Science & Technology, University of California, Davis , 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis , 5UC Davis Comprehensive Cancer Center, University of California, Davis , 6Department of Biological Chemistry, University of California, Davis

Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Direct Infusion Device for Molecule Delivery in Plants

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Imaging ATG9A, a Multi-Spanning Membrane Protein

article

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

article

Author Spotlight: The Production of Recombinant Proteins

article

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

article

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved