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Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

DOI :

10.3791/57498-v

April 26th, 2018

April 26th, 2018

9,476 Views

1Department of Molecular Biology, UT Southwestern Medical Center

Here we use two-dimensional semi-denaturing agarose gel electrophoresis to confirm the presence of amyloid-like fibers of heterogeneous size and exclude the possibility that the size heterogeneity is due to dissociation of the amyloid fibers during the gel running process.

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Keywords 2D Semi denaturing Detergent Agarose Gel Electrophoresis

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