Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

12.8K Views

08:12 min

September 28th, 2018

DOI :

10.3791/58369-v

September 28th, 2018


Transcript

Explore More Videos

Thylakoid Membrane

Chapters in this video

0:04

Title

0:35

Preparing the Blue Native (BN) Gel

3:09

Thylakoid Solubilization

4:19

BN-PAGE

5:05

2D-SDS-PAGE

6:18

Results: Protein Complex Pattern and the Composition of Thylakoid Protein Complexes Solubilized with β-DM and Digitonin

7:25

Conclusion

Related Videos

article

08:55

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

10.1K Views

article

08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

8.7K Views

article

09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

13.7K Views

article

08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

13.9K Views

article

06:36

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

11.8K Views

article

07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

14.0K Views

article

09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

19.6K Views

article

08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

11.2K Views

article

07:54

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

5.9K Views

article

08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

11.2K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved