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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

DOI :

10.3791/57749-v

August 22nd, 2018

August 22nd, 2018

5,833 Views

1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig

This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers.

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Keywords Intrinsic Disorder

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