Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

DOI :

10.3791/57652-v

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11:07 min

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August 26th, 2018

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8,418 Views

Michaela Hoenigsberger¹, Alexey G. Kopchinskiy², Alexandra Parich¹, Karsten Hiller^3,4, Alice Laciny⁵, Herbert Zettel⁵, Linda B.L. Lim⁶, Kamariah A. Salim⁷, Irina S. Druzhinina², Rainer Schuhmacher¹

¹Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Austria, ²Institute of Chemical, Environmental and Biological Engineering, TU Vienna, Austria, ³Department of Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Germany, ⁴Institute for Biochemistry, Biotechnology and Bioinformatics, University of Technology (TU) Braunschweig, Germany, ⁵2nd Zoological Department, Natural History Museum Vienna, Austria, ⁶Chemical Sciences, Universiti Brunei Darussalam, Brunei, ⁷Environmental and Life Sciences, Universiti Brunei Darussalam, Brunei

Chapters

Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described.

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