JoVE Logo
Faculty Resource Center

Sign In

Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector (Video) | JoVE

DOI :

10.3791/57652-v

11:07 min

August 26th, 2018

August 26th, 2018

8,380 Views

1Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Austria, 2Institute of Chemical, Environmental and Biological Engineering, TU Vienna, Austria, 3Department of Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Germany, 4Institute for Biochemistry, Biotechnology and Bioinformatics, University of Technology (TU) Braunschweig, Germany, 52nd Zoological Department, Natural History Museum Vienna, Austria, 6Chemical Sciences, Universiti Brunei Darussalam, Brunei, 7Environmental and Life Sciences, Universiti Brunei Darussalam, Brunei

Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described.

Tags

Isolation

-- Views

Related Videos

article

Assessment of Methane and Nitrous Oxide Fluxes from Paddy Field by Means of Static Closed Chambers Maintaining Plants Within Headspace (Video) | JoVE

article

Laboratory and Field Protocol for Estimating Sheet Erosion Rates from Dendrogeomorphology (Video) | JoVE

article

Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers (Video) | JoVE

article

Combining Eye-tracking Data with an Analysis of Video Content from Free-viewing a Video of a Walk in an Urban Park Environment (Video) | JoVE

article

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles (Video) | JoVE

article

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans (Video) | JoVE

article

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices (Video) | JoVE

article

A Guide to Concentration Alternating Frequency Response Analysis of Fuel Cells (Video) | JoVE

article

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis (Video) | JoVE

article

Ecotoxicological Effects of Microplastics on Bird Embryo Development by Hatching without Eggshell (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved