Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles

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08:42 min

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September 27th, 2024

DOI :

10.3791/67149-v

September 27th, 2024

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Jorge M. S. Faria¹^,², A. Cristina Figueiredo³^,⁴, Dora M. Teixeira⁵, Maria L. Inácio¹^,²

¹INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., ²GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), ³Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁴Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁵Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School, University of Évora

Summary

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Pinewood Nematode

Bursaphelenchus Xylophilus

Environmental Variability

Extraction Techniques

Chapters in this video

0:00

Introduction

1:09

In Vitro Cultivation and Maintenance of Pinewood Nematodes Using Botrytis cinerea

2:40

In Vivo Infection Process of Pinus pinaster Seedlings with Pinewood Nematodes

4:11

Establishment and Infection of In Vitro Pinus pinaster Shoot Cultures with Pinewood Nematodes

6:57

Isolation of Volatile Compounds from Pinus pinaster Seedlings Using Headspace Trapping with Packed Tubes

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