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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

DOI :

10.3791/54582-v

December 14th, 2016

December 14th, 2016

14,722 Views

1Graduate Institute of Pharmacology, College of Medicine, National Taiwan University

Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.

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ELISpot

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