August 31st, 2018
•The aphid Aphis nerii colonizes on highly-defended plants in the dogbane family (Apocyanaceae) and provides numerous opportunities to study plant-insect interactions. Here, we present a series of protocols for the maintenance of plant and aphid cultures, and the generation and analysis of molecular and -omic data for A. nerii.
Related Videos
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved