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Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

DOI :

10.3791/58047-v

October 9th, 2018

October 9th, 2018

9,107 Views

1Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research, Children's Hospital of Philadelphia, 2Division of Plastic Reconstructive Surgery, Center for Fetal Research, Children's Hospital of Philadelphia

We describe a protocol for performing an in utero transplantation (IUT) through intravenous and intra-amniotic routes of injection in the murine model. This protocol can be used to introduce cells, viral vectors, and other substances into the unique immune-tolerant fetal environment.

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Intravenous

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