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Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

DOI :

10.3791/63148-v

10:49 min

February 14th, 2022

February 14th, 2022

2,414 Views

1Department of Cell and Molecular Biology, Karolinska Institutet

In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.

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NEPTUNE

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