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In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

DOI :

10.3791/58083-v

August 24th, 2018

August 24th, 2018

8,366 Views

1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.

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