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Neutrophil Extracellular Traps: How to Generate and Visualize Them

DOI :

10.3791/1724-v

14:17 min

February 24th, 2010

February 24th, 2010

84,428 Views

1Core Facility Microscopy, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.

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Neutrophil Extracellular Traps

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