JoVE Logo
Faculty Resource Center

Sign In

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

DOI :

10.3791/59150-v

9:59 min

January 29th, 2019

January 29th, 2019

9,468 Views

1Department of Nephrology, Leiden University Medical Center

This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.

Tags

High throughput Assay

-- Views

Related Videos

article

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

article

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

article

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

article

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

article

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

article

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

article

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

article

Author Spotlight: Neutrophil Extracellular Traps Imaging in Human and Mouse Tissues

article

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

article

Author Spotlight: Balancing Speed, Cost, and Accuracy in Neutrophil Function Assessment with NeutroFun Screen Protocol

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved