Peripheral Blood Collection and Polymorphonuclear Neutrophil Purification
4:11
Neutrophil Morphology and Viability
7:28
CFSE Staining of Microorganisms and NET Induction
11:10
Results: Analysis of NET Induced in Human Blood Derived Neutrophils
13:19
Conclusion
Transcript
This protocol demonstrates the heterogeneity in composition, structure, and morphology of neutrophil extracellular traps, depending on their inducing stimulus under comparable in vitro conditions in human neutrophils from healthy individuals. High
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Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.