JoVE Logo
Faculty Resource Center

Sign In

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

DOI :

10.3791/64522-v

November 4th, 2022

November 4th, 2022

1,883 Views

1Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca

Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.

-- Views

Related Videos

article

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

article

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

article

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

article

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

article

Granulocyte-dependent Autoantibody-induced Skin Blistering

article

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

article

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

article

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

article

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

article

Author Spotlight: Neutrophil Extracellular Traps Imaging in Human and Mouse Tissues

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved