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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

DOI :

10.3791/58169-v

8:56 min

August 16th, 2018

August 16th, 2018

15,992 Views

1John Innes Centre

Transient heterologous expression of biosynthetic enzymes in Nicotiana benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products. Herein is described a detailed protocol for rapid (5 days) preparative-scale production of triterpenes and analogs utilizing this powerful plant-based platform.

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Transient Expression

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