January 16th, 2021
•We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana benthamiana. This protocol can be extended to purification and visualization of numerous proteins that utilize column chromatography. Moreover, the protocol is adaptable to the in-person and virtual college teaching laboratory, providing project-based exploration.
Related Videos
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved