JoVE Logo
Faculty Resource Center

Sign In

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

DOI :

10.3791/50521-v

July 23rd, 2013

July 23rd, 2013

46,918 Views

1The College of Technology and Innovation, Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University

Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.

-- Views

Related Videos

article

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

article

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

article

High-throughput Purification of Affinity-tagged Recombinant Proteins

article

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

article

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

article

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

article

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

article

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

article

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

article

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved