May 2nd, 2018
•Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.
Tags
Related Videos
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved