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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

DOI :

10.3791/57593-v

May 2nd, 2018

May 2nd, 2018

10,293 Views

1Department of Biomedical Sciences, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University

Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.

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Whole Mount Immunofluorescence

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