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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

DOI :

10.3791/55134-v

January 13th, 2017

January 13th, 2017

10,476 Views

1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

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Organ Culture

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