A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
7.4K Views
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07:15 min
•
August 19th, 2018
DOI :
August 19th, 2018
•Chapters in this video
0:04
Title
1:10
Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
2:25
Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
3:16
Fixing and Staining of Constricted S2R+ Cells
5:18
Imaging and Quantification of the Cellular Contractility Assay
5:41
Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
6:40
Conclusion
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