A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

7.4K Views

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07:15 min

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August 19th, 2018

DOI :

10.3791/58325-v

August 19th, 2018

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Kimberly A. Peters¹, Elizabeth Detmar¹, Liz Sepulveda², Corrina Del Valle², Ruth Valsquier², Anna Ritz², Stephen L. Rogers¹^,³^,⁴, Derek A. Applewhite²

¹Department of Biology, The University of North Carolina at Chapel Hill, ²Department of Biology, Reed College, ³Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, ⁴Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill

Summary

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Keywords Non muscle Myosin II

Contractility

Folded gastrulation Signaling Pathway

Chapters in this video

0:04

Title

1:10

Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells

2:25

Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay

3:16

Fixing and Staining of Constricted S2R+ Cells

5:18

Imaging and Quantification of the Cellular Contractility Assay

5:41

Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction

6:40

Conclusion

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