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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

DOI :

10.3791/58587-v

June 3rd, 2019

June 3rd, 2019

7,229 Views

1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden

This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

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