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High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

DOI :

10.3791/58434-v

6:47 min

September 10th, 2018

September 10th, 2018

7,346 Views

1MRC-Laboratory of Molecular Biology, Cambridge, UK

Macropinocytosis, large-scale non-specific fluid uptake, is important in many areas of clinical biology including immunology, infection, cancer, and neurodegenerative diseases. Here, existing techniques have been adapted to allow high-throughput, single-cell resolution measurement of macropinocytosis in the macropinocytosis model organism Dictyostelium discoideum using flow cytometry.

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High throughput

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