Results: Quantification of Macropinocytosis and Dose-Response Curve for Macropinocytosis Inhibitors
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Conclusion
Transcript
Macropinocytosis is an endocytic pathway that is important for a variety of cellular processes, including cancer metabolism. This protocol allows you to quantify the extent of macropinocytosis in cells in vitro. The automation is aimed at maximizi
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Automated assays using multi-well microplates are advantageous approaches for identifying pathway regulators by allowing the assessment of a multitude of conditions in a single experiment. Here, we have adapted the well-established macropinosome imaging and quantification protocol to a 96-well microplate format and provide a comprehensive outline for automation using a multi-mode plate reader.