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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

DOI :

10.3791/54587-v

October 23rd, 2016

October 23rd, 2016

10,693 Views

1Department of Biology, The Johns Hopkins University, 2Department of Biological Sciences, Duquesne University

Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.

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Keywords PHluorin

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