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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

DOI :

10.3791/58627-v

10:21 min

February 1st, 2019

February 1st, 2019

7,871 Views

1Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, 2TransSINE Technologies Co. Ltd.

SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.

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Cell Based Assays

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