February 1st, 2019
•SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.
Tags
Related Videos
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved