April 10th, 2018
•This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.
Related Videos
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
CRISPR Guide RNA Cloning for Mammalian Systems
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved