Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

8.8K Views

13:04 min

March 1st, 2019

DOI :

10.3791/59233-v

March 1st, 2019


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Long Noncoding RNAs

Chapters in this video

0:04

Title

0:40

Vector Design and Generation

1:52

Virus Generation

4:24

Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit

7:07

dCas9 and gRNA Transduction, Selection, and Clone Creation

10:18

Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA

12:08

Conclusion

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