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JoVE Journal

Bioengineering

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Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

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06:15 min

January 30th, 2019

January 30th, 2019

14,229 Views

0:04

Title

0:32

Turbidity Limulus Amoebocyte Lysate (LAL) Assay

2:26

Sample Loading and Preparation and Gel-Clot LAL

4:27

Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays

5:12

Conclusion

Transcript

This method can help answer in the medical field about safety of nano medicines. The advantages of this technique is that it is standard, and is used worldwide for evaluation of products, To nano medicines. Demonstration of the procedure, will be

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Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles.

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