February 28th, 2019
•This protocol describes the use of single chain MHC class I complexes to investigate molecular interactions in human CD8+ T cell activation: generation of engineered antigen presenting cells expressing single chain constructs, culture of human CD8+ T cell clone and T cell activation experiments.
Tags
Related Videos
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
piggyBac Transposon System Modification of Primary Human T Cells
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved