Retroviral Transduction of T-cell Receptors in Mouse T-cells

34.6K Views

10:14 min

October 22nd, 2010

DOI :

10.3791/2307-v

October 22nd, 2010


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Retroviral Transduction

Chapters in this video

0:00

Title

1:37

Retroviral Transfection and T-cell Isolation

3:55

Prepapration of a Single-cell Suspension from Mouse Spleen

4:51

Isolation and Activation of Mouse T-cells

6:27

Infection of Activated T-cells

8:21

Expansion of Cells and Evaluation of Transduction Efficiency

8:55

Retroviral Expression of TCR on Mouse T-cells

9:32

Conclusion

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