Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
8.1K Views
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08:16 min
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June 6th, 2019
DOI :
June 6th, 2019
•Leighton H. Duncan1,5, Mark W. Moyle1,5, Lin Shao1,5, Titas Sengupta1,5, Richard Ikegami1,5, Abhishek Kumar4,5, Min Guo4,5, Ryan Christensen4,5, Anthony Santella2,5, Zhirong Bao2,5, Hari Shroff4,5, William Mohler3,5, Daniel A. Colón-Ramos1,5,6
1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico
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Chapters in this video
0:04
Title
0:34
C. elegans Embryo Sample Mounting Preparation
1:48
Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
3:12
Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
6:06
Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
7:43
Conclusion
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