Detection of Telomerase Extension Products and Data Analysis
5:03
Results: Telomerase Activity
5:51
Conclusion
Transcript
The ddTRAP assay provides the ability to obtain a sensitive, robust, and highly quantitative measurement of telomerase enzymatic activity in cells. The ability to run up to 96 samples per run in a medium-throughput fashion offers us a great advant
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We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.