JoVE Logo
Faculty Resource Center

Sign In

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

DOI :

10.3791/59895-v

July 5th, 2019

July 5th, 2019

9,250 Views

1Department of Pathology, University of Colorado - Anschutz Medical Campus

This article details the use of an anchored multiplex polymerase chain reaction-based library preparation kit followed by next-generation sequencing to assess for oncogenic gene fusions in clinical solid tumor samples. Both wet-bench and data analysis steps are described.

Tags

Oncogenic Gene Fusion Detection

-- Views

Related Videos

article

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

article

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

article

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

article

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

article

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

article

Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

article

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

article

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

article

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

article

Transmitochondrial Cybrid Generation Using Cancer Cell Lines (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved