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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

DOI :

10.3791/59593-v

September 5th, 2019

September 5th, 2019

7,056 Views

1Blood Cell Development and Function Program, Fox Chase Cancer Center

We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

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Flow Cytometric Analysis

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