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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

DOI :

10.3791/56976-v

March 26th, 2018

March 26th, 2018

13,479 Views

1Laboratoire d’hématologie, CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille

We outline a protocol to detect simultaneous expression of leukemia stem cell markers on primary acute myeloid leukemia cells by flow cytometry. We show how to quantify three progenitor populations and a putative LSC population with increasing degree of maturation. We confirmed the presence of these populations in corresponding patient-derived-xenografts.

Tags

Flow Cytometry

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