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Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

DOI :

10.3791/58642-v

January 26th, 2019

January 26th, 2019

9,483 Views

1School of Life Sciences, The Chinese University of Hong Kong, 2State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, 3Key Laboratory for Regenerative Medicine, Ministry of Education, The Chinese University of Hong Kong, 4Centre for Novel Biomaterials, The Chinese University of Hong Kong

Here, we present a protocol to isolate apoptotic breast cancer cells by fluorescence-activated cell sorting and further detect the transition of breast non-stem cancer cells to breast cancer stem cell-like cells after apoptosis reversal by flow cytometry.

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Flow Cytometric Detection

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