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Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

DOI :

10.3791/64629-v

February 10th, 2023

February 10th, 2023

981 Views

1Stem Cells and Cancer Laboratory, Vall d’Hebron Institute of Oncology (VHIO), 2Preclinical Therapeutics Core, University of California San Francisco, 3CIBERONC

This protocol describes the orthotopic implantation of patient-derived cancer cells in the cecum wall of immunodeficient mice. The model recapitulates advanced colorectal cancer metastatic disease and allows for the evaluation of new therapeutic drugs in a clinically relevant scenario of lung and liver metastases.

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Orthotopic Implantation

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