JoVE Logo
Faculty Resource Center

Sign In

Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease

DOI :

10.3791/59597-v

6:52 min

July 6th, 2019

July 6th, 2019

8,662 Views

1Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health, University of Manchester, 2Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester

This protocol illustrates a cell encapsulation method by rapid physical gelation of alginate to immobilize cells. Obtained microbeads allow controlled and sustained secretion of amyloid-β over time and can be used to study the effects of secreted amyloid-β in in vitro and in vivo models.

Tags

Fabrication

-- Views

Related Videos

article

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

article

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

article

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

article

The use of Biofeedback in Clinical Virtual Reality: The INTREPID Project

article

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

article

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

article

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

article

Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction

article

The Use of Chemostats in Microbial Systems Biology

article

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved