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Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

DOI :

10.3791/4027-v

June 19th, 2012

June 19th, 2012

12,184 Views

1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.

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SecM Arrest Sequence

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