September 14th, 2019
•We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.
Related Videos
Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved