JoVE Logo
Faculty Resource Center

Sign In

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

DOI :

10.3791/58407-v

8:36 min

November 1st, 2018

November 1st, 2018

30,934 Views

1Department of Microbiology and Infection Control, Osaka Medical College

Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

Tags

Dengue Virus RNA

-- Views

Related Videos

article

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

article

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

article

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

article

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

article

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

article

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

article

Real-time Live Imaging of T-cell Signaling Complex Formation

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved