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Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

DOI :

10.3791/51207-v

8:11 min

September 1st, 2015

September 1st, 2015

8,319 Views

1Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Microbiology, Infectiology, and Immunology, Université de Montréal

Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.

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Innate Sensing

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