August 14th, 2019
•The characterization of circulating tumor cells (CTCs) is a popular topic in translational research. This protocol describes a semi-automatic immunofluorescence (IF) assay for PD-L1 characterization and enumeration of CTCs in non-small cell lung cancer (NSCLC) patient samples.
Related Videos
Lung Tumor Cell Recruitment Assay (Video) | JoVE
Capture and Release of Viable Circulating Tumor Cells from Blood (Video) | JoVE
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer (Video) | JoVE
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues (Video) | JoVE
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH (Video) | JoVE
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts (Video) | JoVE
Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients (Video) | JoVE
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression (Video) | JoVE
Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions (Video) | JoVE
Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved